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mouse derived neuroblastoma cell line neuro 2a  (ATCC)


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    Structured Review

    ATCC mouse derived neuroblastoma cell line neuro 2a
    Mouse Derived Neuroblastoma Cell Line Neuro 2a, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4241 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+neuroblastoma+cell+line/Neuro-2a/pm42296711-47-1-10
    Average 99 stars, based on 4241 article reviews
    mouse derived neuroblastoma cell line neuro 2a - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Activity Assay:

    Article Title: Design and Synthesis of New Quinoxaline Analogs to Regenerate Sensory Auditory Hair Cells
    Article Snippet: .. Additionally, we assessed the activity of Qx-derived compounds in the mouse neuroblastoma cell line, Neuro-2A, and the human fibrosarcoma HT-1080 (ATCC, Manassas, VA, USA) following the standard cell culture guidelines provided by the vendor. ..

    Cell Culture:

    Article Title: Design and Synthesis of New Quinoxaline Analogs to Regenerate Sensory Auditory Hair Cells
    Article Snippet: .. Additionally, we assessed the activity of Qx-derived compounds in the mouse neuroblastoma cell line, Neuro-2A, and the human fibrosarcoma HT-1080 (ATCC, Manassas, VA, USA) following the standard cell culture guidelines provided by the vendor. ..

    Article Title: Aptamer-Encapsulated Cellular Nanoparticles for Neurotoxin Neutralization.
    Article Snippet: Aptamers are single-stranded oligonucleotides that fold into defined architectures for specific target binding.. In this study, aptamers are selected that specifically bind to small-molecule neurotoxins and encapsulate them into cell membrane-coated nanoparticles (referred to as ‘cellular nanoparticles’ or ‘CNPs’) for effective neutralization of neurotoxins.. Specifically, six different aptamers are selected that bind to saxitoxin (STX) or tetrodotoxin (TTX) and encapsulate them into metal–organic framework cores, which are then coated with neuronal cell membrane.

    Article Title: IntAct-U-ExM: Ultrastructure Expansion microscopy of actin networks via an internally-tagged actin
    Article Snippet: .. Neuro-2a cell line, a mouse neuroblastoma cell line (ATCC® CCL-131TM, RRID: CVCL_0470) was cultured at 37 °C and 5% CO2 in Dulbecco modified Eagle medium (Gibco 12430-054) with 100 U Penicillin/mL and 0.1 mg Streptomycin/mL (Pen Strep Gibco 15140-122), 10% Fetal Bovine Serum (FBS; HIMEDIA RM10434), and 1 % Glutamax (Gibco 35050/061). .. For transfection, 6 × 10 cells/well were seeded on 18-mm coverslips in a 12-well plate and grown overnight in complete DMEM after which, the media was changed to pre-filtered DMEM without antibiotics. the cells were transfected with 20 ng of either β-IntAct or γ-IntAct using LipofectamineTM 3000 Transfection Reagent (#L3000075, Thermo Scientific) where the plasmid DNA was diluted in 50 μl of Opti MEM Reduced Serum Medium (#31985070, Thermo Scientific) along with 1 μl of P3000 reagent.

    Article Title: Lactate phosphorylates nNOS Ser1412 and protects against restraint stress-induced memory impairment in mice.
    Article Snippet: Lactate is a potential energy source in the brain and contributes to several cognitive functions, such as learning, memory, stress resilience, and mood regulation; However, the mechanisms by which lactate contributes to stressinduced cognitive dysfunction, including the roles of nitric oxide (NO) signaling, remain unclear.. We hypothesized that lactate improves cognitive dysfunction induced by acute restraint stress via neuronal NO synthase (nNOS), a major NO source in the central nervous system, and the brain-derived neurotrophic factor (BDNF) pathway.. We demonstrated that lactate phosphorylates nNOS at Ser1412 and promotes the neural differentiation of Neuro2a, a mouse neuroblastoma cell line.

    Article Title: Dual-Modal Cellular Nanoparticles for Continuous Neurotoxin Detoxification
    Article Snippet: .. Neuro-2a cells, a mouse neuroblastoma cell line obtained from the ATCC (CCL-131), were cultured following the 3 same procedure described above. ..

    Modification:

    Article Title: Two phosphomimetic sites in the GABA transporter GAT1 C-terminus selectively regulate transporter interactions with distinct types of PDZ domains in vitro.
    Article Snippet: The GABA transporter GAT1 is a membrane protein that participates in GABA signalling in the brain and certain peripheral tissues.. GAT1 contains a C-terminal PDZ motif (-A597Y598I599), previously classified as a type II (ΦXΦ) motif, where PDZ interaction should depend on non-phosphorylatable hydrophobic (Φ) residues at PDZ positions 0 (I599) and − 2 (A597).. We recently found that a GAT1 C-terminal peptide unconventionally binds to the PDZ1 domain of syntenin-1 and to the PDZ2 domain of PSD95 by using different PDZ interaction schemes, specifically 0,-1 and 0,-3, respectively.

    Article Title: IntAct-U-ExM: Ultrastructure Expansion microscopy of actin networks via an internally-tagged actin
    Article Snippet: .. Neuro-2a cell line, a mouse neuroblastoma cell line (ATCC® CCL-131TM, RRID: CVCL_0470) was cultured at 37 °C and 5% CO2 in Dulbecco modified Eagle medium (Gibco 12430-054) with 100 U Penicillin/mL and 0.1 mg Streptomycin/mL (Pen Strep Gibco 15140-122), 10% Fetal Bovine Serum (FBS; HIMEDIA RM10434), and 1 % Glutamax (Gibco 35050/061). .. For transfection, 6 × 10 cells/well were seeded on 18-mm coverslips in a 12-well plate and grown overnight in complete DMEM after which, the media was changed to pre-filtered DMEM without antibiotics. the cells were transfected with 20 ng of either β-IntAct or γ-IntAct using LipofectamineTM 3000 Transfection Reagent (#L3000075, Thermo Scientific) where the plasmid DNA was diluted in 50 μl of Opti MEM Reduced Serum Medium (#31985070, Thermo Scientific) along with 1 μl of P3000 reagent.

    Article Title: Lactate phosphorylates nNOS Ser1412 and protects against restraint stress-induced memory impairment in mice.
    Article Snippet: Lactate is a potential energy source in the brain and contributes to several cognitive functions, such as learning, memory, stress resilience, and mood regulation; However, the mechanisms by which lactate contributes to stressinduced cognitive dysfunction, including the roles of nitric oxide (NO) signaling, remain unclear.. We hypothesized that lactate improves cognitive dysfunction induced by acute restraint stress via neuronal NO synthase (nNOS), a major NO source in the central nervous system, and the brain-derived neurotrophic factor (BDNF) pathway.. We demonstrated that lactate phosphorylates nNOS at Ser1412 and promotes the neural differentiation of Neuro2a, a mouse neuroblastoma cell line.

    Transfection:

    Article Title: Two phosphomimetic sites in the GABA transporter GAT1 C-terminus selectively regulate transporter interactions with distinct types of PDZ domains in vitro.
    Article Snippet: The GABA transporter GAT1 is a membrane protein that participates in GABA signalling in the brain and certain peripheral tissues.. GAT1 contains a C-terminal PDZ motif (-A597Y598I599), previously classified as a type II (ΦXΦ) motif, where PDZ interaction should depend on non-phosphorylatable hydrophobic (Φ) residues at PDZ positions 0 (I599) and − 2 (A597).. We recently found that a GAT1 C-terminal peptide unconventionally binds to the PDZ1 domain of syntenin-1 and to the PDZ2 domain of PSD95 by using different PDZ interaction schemes, specifically 0,-1 and 0,-3, respectively.



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    ATCC neuro 2a n2a mouse neuroblastoma cell lines
    Abcam ab64186 recognizes NRN1 protein in mouse <t>neuroblastoma</t> cells. (A) Representative Western blot of human embryonic kidney (HEK) <t>293T,</t> <t>Neuro‐2a</t> <t>(N2a)</t> mouse neuroblastoma, and SH‐SY5Y human neuroblastoma cell lysates (50 µg protein), probed with NRN1 polyclonal antibody Abcam ab64186 and GAPDH monoclonal antibody. (B) N2a cells were transfected with NRN1 or Scramble (non‐targeting) siRNA smart pools and harvested after 96 h. Western blot analyses with NRN1 polyclonal antibody Abcam ab64186 revealed reduced intensity of the ∼34 kDa band in NRN1‐depleted cells, compared to Scramble control. 10 µg of protein was loaded per lane, and GAPDH was probed as a loading control. GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; NRN1, Neuritin‐1; siRNA, small interfering RNA;
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    Abcam ab64186 recognizes NRN1 protein in mouse neuroblastoma cells. (A) Representative Western blot of human embryonic kidney (HEK) 293T, Neuro‐2a (N2a) mouse neuroblastoma, and SH‐SY5Y human neuroblastoma cell lysates (50 µg protein), probed with NRN1 polyclonal antibody Abcam ab64186 and GAPDH monoclonal antibody. (B) N2a cells were transfected with NRN1 or Scramble (non‐targeting) siRNA smart pools and harvested after 96 h. Western blot analyses with NRN1 polyclonal antibody Abcam ab64186 revealed reduced intensity of the ∼34 kDa band in NRN1‐depleted cells, compared to Scramble control. 10 µg of protein was loaded per lane, and GAPDH was probed as a loading control. GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; NRN1, Neuritin‐1; siRNA, small interfering RNA;

    Journal: Alzheimer's & Dementia

    Article Title: NRN1 as a therapeutic target for Alzheimer's disease

    doi: 10.1002/alz.71149

    Figure Lengend Snippet: Abcam ab64186 recognizes NRN1 protein in mouse neuroblastoma cells. (A) Representative Western blot of human embryonic kidney (HEK) 293T, Neuro‐2a (N2a) mouse neuroblastoma, and SH‐SY5Y human neuroblastoma cell lysates (50 µg protein), probed with NRN1 polyclonal antibody Abcam ab64186 and GAPDH monoclonal antibody. (B) N2a cells were transfected with NRN1 or Scramble (non‐targeting) siRNA smart pools and harvested after 96 h. Western blot analyses with NRN1 polyclonal antibody Abcam ab64186 revealed reduced intensity of the ∼34 kDa band in NRN1‐depleted cells, compared to Scramble control. 10 µg of protein was loaded per lane, and GAPDH was probed as a loading control. GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; NRN1, Neuritin‐1; siRNA, small interfering RNA;

    Article Snippet: Neuro‐2a (N2a) mouse neuroblastoma cell lines (Catalog No.: CCL‐131, ATCC) were maintained in MEM (Catalog No.: 11095‐080, Thermo Fisher Scientific) with 10% FBS and 1% penicillin‐streptomycin.

    Techniques: Western Blot, Transfection, Control, Small Interfering RNA